Application of Milk Permeate as an Inducer for the Production of Microbial Recombinant Lipolytic Enzymes

Recombinantly produced enzymes are applied in many fields, ranging from medicine to food and nutrition, production of detergents, textile, leather, paper, pulp, plastics. Thus, the cost-effectiveness recombinant enzyme synthesis is an important issue biotechnological industry. Isopropyl-β-D-thiogalactoside (IPTG), analog lactose, currently most widely used chemical agent for induction synthesis. However, use IPTG can lead toxic elements introduce physiological stress cells. this study aims find a simpler, cheaper, safer way produce enzymes. In study, several previously designed lipolytic (GDEst-95 esterase, GD-95RM lipase, fused GDEst-lip enzyme, putative cutinase Cut+SP Streptomyces scabiei 87.22) induced E. coli BL21 (DE3) using 4 mM milk permeate, type waste manufacturing process possessing >82% lactose. The SDS-PAGE analysis clearly indicates all target during 2–12 h post-induction timeframe. Further investigation GDEst-95, GD-95RM, GDEst-lip, biocatalysts was carried out spectrophotometrically zymography method, confirming fully active